TABLE 4.
Env construct | Titer (AP+ FFU/ml) for indicated target cells:
|
|||
---|---|---|---|---|
BEAS-2B
|
CF16
|
|||
Lentiviral vector | Oncoretroviral vector | Lentiviral vector | Oncoretroviral vector | |
JSRV Env | 3.3 × 104 | 2.8 × 104 | 9.0 × 103 | 1.4 × 104 |
JSRV-HIV | 1.8 × 104 | 4.0 × 102 | 7.2 × 103 | 2.9 × 102 |
JSRV-10A1 | 1.8 × 103 | 3.0 × 102 | 6.2 × 102 | 51 |
JSRV-10A1R− | 3.8 × 103 | 2.9 × 103 | 6.1 × 103 | 4.5 × 102 |
VSV-G | 5.9 × 104 | 1.5 × 106 | 8.8 × 103 | 1.2 × 105 |
Experimental approaches were the same as those described in Table 1. The titers on BEAS-2B human lung epithelial cells (43) and CF16 cells established from a CF patient (18) were determined and shown. The values shown are the means of the results of two independent experiments, with variations of no more than 10%.