FIG. 4.
Yeast three-hybrid interaction assay. Yeast strain CG1945 was cotransfected with pBridgeBD-KLC-TPR/A36R24-111 and pADA33R1-40. (A) Extracts from cotransformed yeast that had been grown in medium with (+) or without (−) methionine were analyzed by SDS-PAGE followed by Western blotting with anti-HA MAb. The masses (in kilodaltons) and positions of migration of markers are indicated on the left. (B) Tenfold dilutions of overnight cultures of cotransformed yeast were applied as spots to selective medium with (+) or without (−) methionine and histidine.