Table 2.
Serum and liver levels of β-carotene, retinal, APO-8′-, APO-10′-, APO-12′- and APO-14′-carotenals for β-carotene fed wild type and Bcmo1-deficient mice.a
| β-Carotene | Retinal | Apo-8′-carotenal | Apo-10′-carotenal | Apo-12′-carotenal | Apo-14′-carotenal | |
|---|---|---|---|---|---|---|
| Serum (nM) | ||||||
| Wild type (n = 5) | 1.9 (0.9–2.6) | N.D. | N.D. | 0.2 (0.1–0.5) | 0.4 (0.1–1.1) | N.D. |
| Bcmo1−/− (n = 3) | 80.8 (73.5–89.3)b | N.D. | N.D. | 0.5 (0.4–0.7) | 0.7 (0.6–0.8) | N.D. |
| Liver (pmol/g) | ||||||
| Wild type (n = 5) | 16.8 (6.3–27.9) | 356 (198–440) | N.D. | 0.2 (0.0–0.8) | 5.9 (4.2–8.5) | N.D. |
| Bcmo1−/− (n = 4) | 381 (241–670)b | 408 (159–656) | N.D. | 0.8 (0.0–1.9) | 12.0 (7.4–16.3)c | N.D. |
| Diet (nmol/g) (n = 3) | 19.7 ± 4.2 | N.D. | 0.1 ± 0.0 | 0.2 ± 0.1 | 0.2 ± 0.1 | 0.3 ± 0.1 |
a
Serum and liver concentrations are given as the mean followed in parenthesis by the range of values measured for all samples from that group. Concentrations of compounds in the diet are provided as the means ± S.D. Limits of detection for apo-carotenals range from approximately 0.1 to 0.5 nM for serum and 0.1 to 0.7 pmol/g liver. Limits of detection for retinal in serum are approximately 40–50 nM. N.D. signifies not detected.
b
Significantly different from corresponding wild type, p < 0.01.
c
Significantly different from corresponding wild type, p < 0.05.