Fig. 3.

Changes in sympathetic innervation after clodronate liposome treatment are reflected by multiple markers. Adjacent sections from hearts receiving coronary artery ligation plus saline (C+S) or ligation plus clodronate liposomes (C+C) were immunostained for DBH, which characterizes noradrenergic neurons (a, d), the intermediate filament protein peripherin which stains intact unmyelinated axons (b, e), and tyrosine hydroxylase that is a marker for catecholaminergic axons (c, f). Levels of innervation density were comparable under different treatments with all markers. Scale bar 10 μm