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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Acta Neuropathol. 2013 Jan 31;125(4):581–593. doi: 10.1007/s00401-013-1080-2

Figure 1.

Figure 1

(a) Location and sequence of antigen used to generate an antibody targeting acetylated tau at lysine 274. (b) MAb 359 recognizes both 4R and 3R tau. HEK293 cells were transfected with either 4R or 3R tau in the presence or absence of p300 overexpression. MAb 359 selectively recognized p300-induced acetylation in both 4R and 3R tau. Total tau level was detected with Tau5 antibody. (c) MAb 359 is specific for ac-K274. HEK293 cells were transfected with WT or K274R tau in the presence or absence of p300 overexpression. Mutation of K274 abolished p300-induced immunoreactivity of MAb 359.