Table 2. NZSL MHC DRB alleles and frequency in NZSL live pup population as derived from Sanger sequencing and Phase reconstruction.
| Allele number | Alleles—bp position consensus | Count | Frequency | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| |
58
|
59
|
61
|
88
|
148
|
151
|
161
|
168
|
170
|
|
|
| 1 | T | T | G | C | C | C | C | T | C | 1 | 0.57 |
| 2/5 | T | T | G | C | C | C | G | T | A | 1 | 0.57 |
| 3/4 | T | T | G | C | C | A | G | T | A | 43 | 24.71 |
| 6 | T | T | G | C | A | C | C | T | C | 2 | 1.15 |
| 7 | T | T | G | C | T | A | C | T | C | 4 | 2.3 |
| 8 | T | T | G | T | C | C | G | C | A | 3 | 1.72 |
| 9 | T | T | G | T | C | A | A | C | A | 1 | 0.57 |
| 10 | T | T | G | T | C | A | G | C | A | 21 | 12.07 |
| 11 | T | T | G | T | A | C | C | T | C | 1 | 0.57 |
| 12 | T | A | G | C | T | A | C | T | C | 3 | 1.72 |
| 13 | A | T | T | C | A | C | C | T | C | 1 | 0.57 |
| 14 | A | T | T | T | C | C | C | T | A | 2 | 1.15 |
| 15 | A | T | T | T | C | C | G | T | A | 6 | 3.45 |
| 16 | A | T | T | T | C | C | G | T | C | 1 | 0.57 |
| 17 | A | T | T | T | C | A | G | T | A | 1 | 0.57 |
| 18 | A | T | T | T | A | C | C | T | A | 1 | 0.57 |
| 19 | A | T | T | T | A | C | C | T | C | 2 | 1.15 |
| 20 | A | T | T | T | A | C | G | T | A | 2 | 1.15 |
| 21 | A | T | T | T | A | C | G | T | C | 1 | 0.57 |
| 22 | A | A | G | T | C | C | G | C | A | 1 | 0.57 |
| 23 | A | A | T | T | C | C | C | T | C | 2 | 1.15 |
| 24 | A | A | T | T | C | C | G | T | A | 1 | 0.57 |
| 25 | A | A | T | T | C | C | G | T | C | 4 | 2.3 |
| 26 | A | A | T | T | A | C | C | T | C | 67 | 38.51 |
| 27 | A | A | T | T | A | C | G | T | C | 1 | 0.57 |
| 28 | A | A | T | T | G | C | C | T | C | 1 | 0.57 |
The most frequent alleles and those used in further analyses are shaded grey. Base pair (bp) consensus positions are arbitrarily calculated from the start position of the amplified PCR fragment in NZSL DNA. Alleles 2 and 5, and 3 and 4, were synonymous at the amino-acid level, forming two distinct alleles by protein sequence. Here, frequencies are combined, and the most common nucleotide sequence is listed.