Table 2. Summary of QTL associated with soybean aphid resistance identified in the 184 RILs of the Wyandot × PI 567324 population using single-factor ANOVA and composite interval mapping methods.

Phenotype a	Marker b	Chr.	QTL name	Position (bp) c	Single-factor ANOVA		Composite interval mapping
					R2d	P	LOD e	R2d	af
Field2W	BARCSOYSSR_06_0998	6	QTL_6_1	18713522	11.3	<0.0001	4.8	11.6	−0.50
	BARCSOYSSR_13_1139	13	QTL_13_1	29274967	58.1	<0.0001	35.9	42.7	0.99
	Satt649	13	QTL_13_2	12953321	29.7	<0.0001	14.7	13.1	0.55
Field4W	BARCSOYSSR_06_0998	6	QTL_6_1	18713522	7.7	0.0002	3.2	8.0	−0.46
	BARCSOYSSR_13_1139	13	QTL_13_1	29274967	70.3	<0.0001	50.3	54.4	1.23
	Satt649	13	QTL_13_2	12953321	25.3	<0.0001	14.2	9.3	0.51
GH2W	BARCSOYSSR_06_0998	6	QTL_6_1	18713522	5.1	0.0027	2.1	5.4	−0.39
	BARCSOYSSR_13_1139	13	QTL_13_1	29274967	80.6	<0.0001	63.5	70.6	1.43
	Satt649	13	QTL_13_2	12953321	13.4	<0.0001	4.5	2.1	0.25
GH4W	BARCSOYSSR_06_0998	6	QTL_6_1	18713522	4.2	0.0068	1.7	4.4	−0.35
	BARCSOYSSR_13_1139	13	QTL_13_1	29274967	65.1	<0.0001	38.9	56.9	1.30
	Satt649	13	QTL_13_2	12953321	11.4	<0.0001	2.4	2.1	0.25

Abbreviations: ANOVA, analysis of variance; QTL, quantitative trait loci; RIL, recombinant inbred line.

^aField2W: aphid scores after 2 weeks in the field cage, Field4W: aphid scores after 4 weeks in the field cage, GH2W: aphid scores after 2 weeks in the greenhouse, and GH4W: aphid scores after 4 weeks in the greenhouse.

^bMarker that revealed the highest R² value among significant markers.

^cStart position of significant markers is based on the Soybean Consensus Map 4.0 and BARCSOYSSR_1.0 soybean SSR database.

^dPercentage of phenotypic variation explained by the QTL detected in our mapping population.

^eGenome-wide LOD thresholds for 184 RILs is 2.0.

^fAdditive effect. A positive value indicates that resistance alleles were derived from the resistant parent PI 567324, whereas a negative symbol implies that resistance alleles were contributed by the susceptible parent Wyandot.