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Single-cycle infection assays. MAGI-5 cells were infected with WT or mutant viruses with equivalent amounts of p24 in three different dilutions. At 40 h postinfection, cells were fixed and incubated with β-galactosidase substrate. Blue colonies were counted for each mutant and plotted on the y axis. The error bars represent values for triplicate samples from five independent experiments. (A) Single mutations. (B) Multiple mutations.
