Figure 2.

EFV does not induce apoptosis in NHKs. (A) NHKs were exposed to 10 μM EFV for 72 h and subjected to TUNEL assay to detect fragmentation of DNA ends, characteristic of apoptotic cell death. Cells treated with 10 μM cisplatin for 24 h were used as a positive control. (B) NHKs and NHFs exposed to 10 μM EFV were assayed by western blotting to detect cleavage of caspase-3 and PARP. (C) Western blotting showed progressive reduction of CDK2 and cyclin A2 in NHKs exposed to 10 μM EFV. As a control, we included Jurkat cells exposed to 5 Gy ionizing radiation (indicated by IR). β-actin and GAPDH served as loading controls.