Figure 5.

EFV induces terminal differentiation in NHKs. (A) Western blotting was performed with EFV-treated NHKs to detect various proteins as indicated. (B) mRNA expression levels for differentiation-associated genes were determined by qPCR. Error bars, SD. ^*P<0.05 against control. (C) NHKs were exposed to 10 μM EFV for 48 h with or without 3-MA (5 mM) and stained for involucrin by IPS. Original magnification, ×100. (D) Involucrin expression was determined in NHKs exposed to rapamycin (Rapa), EFV or AZT in the absence or presence of 3-MA. E, proliferating cells at PD 12; L, senescent cells at PD 22 were included as controls. β-actin served as a loading control. Inv, involucrin; Fil, filaggrin; Lor, loricrin, K1, keratin 1.