TABLE 3.
Midgut distribution of mature virions after high-dose infection of ticks with Pr4 and Pr4Δ3-C2a
| Time postfeeding and virus strain | No. of phagocytic digestive cells examined | No. of phagocytic digestive cells with virus (no. of virions) | No. of undifferentiated cells examined | No. of undifferentiated cells with virus (no. of virions) | Virus titer in whole tick (log10 HAD50/ml) (mean ± SEM) |
|---|---|---|---|---|---|
| 7 days | |||||
| Pr4 | 300 | 2 (43) | 230 | 0 | 5.4 ± 0.10 |
| Pr4Δ3-C2 | 818 | 14 (106) | 786 | 0 | 4.0 ± 0.26 |
| 21 days | |||||
| Pr4 | 185 | 31 (999) | 760 | 7 (72) | 6.8 ± 0.20 |
| Pr4Δ3-C2 | 398 | 3 (43) | 770 | 0 | 5.0 ± 0.44 |
a
Nymphal ticks (stage N3) were exposed to virus doses of 108 HAD50/ml.