Figure 2. Caffeine attenuated MPTP-induced gliosis.
(A) MPTP significantly (p < 0.001) increased GFAP immunoreactivity in striatum and this effect was blocked significantly (p < 0.001) by caffeine. The upper left panel of four photomicrographs was representative of findings observed in 3 animals with 6 sections taken from each animal. ***p < 0.001, Bar = 50 μm. (B) In addition to microglia, lectin staining also revealed blood vessels. Amoeboid-shaped microglia (arrow heads) were observed only in striatum from MPTP treated animals; not in control or caffeine-treated animals. Representative images were taken from 3 animals; 6 sections were taken from each animal. Bar = 50 μm.