Table 2. Characteristics of primers used for real-time QRT-PCR.
| Gene | Sequence of primers | Length of amplicon (bp) * | Tm (°C) † |
|---|---|---|---|
| SOD2 |
Forward: 5′-CTGATTTGGACAAGCAGCAA-3′ |
199 |
81.6 |
| Reverse: 5′-CTGGACAAACCTCAGCCCTA-3′ |
|||
| ALDH1A1 |
Forward: 5′-TACTCACCGATTTGAAGATT-3′ |
151 |
77.2 |
| Reverse: 5′-TTGTCAACATCCTCCTTATC-3′ |
|||
| MGST1 |
Forward: 5′- ATTGGCCTCCTGTATTCCTTG-3′ |
311 |
80.2 |
| Reverse: 5′-TAATCCCTCTGCTCCCCTCC-3′ |
|||
| GAPDH |
Forward: 5′-GAAGGTGAAGGTCGGAGTC-3′ |
226 |
80.0 |
| Reverse: 5′-GAAGATGGTGATGGGATTC-3′ |
* bp – base pairs † Tm – melting temperature