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. 2013 Jun 25;8(6):e67209. doi: 10.1371/journal.pone.0067209

Figure 5. Stimulation potential of TLCs of female donors younger and older than 65 years of age.

Figure 5

A–B Cell count of TLCs of female donors younger (A) and older (B) than 65 years was measured by Alamar Blue assay and given as percentage relative to untreated controls. BMP-2 application increased cell count at day 7 at the low and high concentration in cells of donors younger than 65 years. High BMP-2 concentration significantly decreased cell count at day 5 in TLCs of donors older than 65 years of age. Application of BMP-7 enhanced cell count at the high concentration at day 5 and 7 in cells of both donor groups. Gray boxes in graphs indicate significant differences between donors </>65 years, while older donors showed a decreased stimulated cell count. C–D Col-I protein synthesis in cell culture supernatant of day 7 after growth factor application of TLCs of female donors younger (C) and older (D) than 65 years of age. Col-I synthesis was calculated relative to total protein and given as percentage to the untreated control. The BMP-2 and BMP-7 treatment of TLCs of both donor groups significantly increased Col-I protein synthesis at all concentrations. E–F Col-I and Col-III gene expression after growth factor application of TLCs of female donors younger (E) and older (F) than 65 years. Gene expression is given as 2−ΔΔCt and was normalized to the untreated control. The Col-I and Col-III expression was significantly increased by high BMP-2 and both BMP-7 concentrations in the female group<65 years. In cells of females >65 years both factors increased the Col-I expression, but Col-III expression was only increase by high concentrations of BMP-2 and BMP-7. The asterisks (*) mark significant differences to the untreated control. The numbers give details for the p-value: 1: p≤0.05; 2: p≤0.001.