Table 2. Primer.
| Gene | Sequence forward primer | Sequence reverse primer | Source | °C |
| Housekeeping genes | ||||
| GAPDH | CCACTCCTCCACCTTTGACG | CATGAGGTCCACCACCCTGT | Primer3 | |
| RPL13 | CCTGGAGGAGAAGAGGAAAGAGA | TTGAAGGACCTCTGTGTATTTGTCAA | Primer3 | |
| Characterization markers | ||||
| Col-I | TGACCTCAAGATGTGCCACT | ACCAGACATGCCTCTTGTCC | Primer3 | 64 |
| Col-III | GCTGGCATCAAAGGACATCG | TGTTACCTCGAGGCCCTGGT | Primer3 | 64 |
| Scleraxis | QuantiTect Primer Assay Kit SCXB (Qiagen, Hilden, Germany) | 60 | ||
| TNMD | TTGAAGACCCACGAAGTAGA | ATGACATGGAGCACACTTTC | [56] | 60 |
| Mohawk | TGGTTTGCTAATGCAAGACG | CCTTCGTTCATGTGGGTTCT | Primer3 | 60 |
| Decorin | CGCCTCATCTGAGGGAGCTT | TACTGGACCGGGTTGCTGAA | Primer3 | 64 |
| TGF-β1 | AAGGACCTCGGCTGGAAGTG | AGGGCCAGGACCTTGCTGTA | Primer3 | 64 |
| TGF-β2 | CAACAGCACCAGGGACTTGC | AGCACAAGCTGCCCACTGAG | Primer3 | 64 |
| TGF-β3 | CTGCTGGAGGAGATGCATGG | GGCAGACAGCCAGTTCGTTG | Primer3 | 64 |
| Adipogenic markers | ||||
| PPARγ | TGCAGTGGGGATGTCTCATA | CAGCGGGAAGGACTTTATGT | Primer3 | 60 |
| LPL | TCCGTGGCTACCTGTCATTT | ACATCCTGTCCCACCAGTTT | Primer3 | 60 |
| FABP4 | TCAGTGTGAATGGGGATGTG | CCACCAGTTTATCATCCTCTCG | Primer3 | 60 |
| Osteogenic markers | ||||
| OC | CCCAGGCGCTACCTGTATCAA | CTGGAGAGGAGCAGAACTGG | Primer3 | 64 |
| ALPL | GGAAATCTGTGGGCATTGTG | CCCTGATGTTATGCATGAGC | Primer3 | 60 |
| Runx2 | GCCCCCAAACAGTATCTTGA | GCCTGAAGTGAGGTTTTAGGC | Primer3 | 60 |
| Chondrogenic markers | ||||
| ACAN | CCAGTGCACAGAGGGGTTTG | TCCGAGGGTGCCGTGAG | [57] | 64 |
| COMP | GCAACACGGACGAGGACAAG | CGCCATCACTGTCCTTCTGG | Primer3 | 64 |
| Col-II | CGCACCTGCAGAGACCTGAA | TCTTCTTGGGAACGTTTGCTGG | Primer3 | 66 |
RPL13: ribosomal protein L13; TNMD: tenomodulin; PPARγ: peroxisome proliferator-activated receptor gamma; FABP4: fatty acid binding protein; LPL: lipoprotein lipase; OC: osteocalcin; ALPL: alkaline phosphatase tissue-nonspecific isozyme; Runx2: runt-related transcription factor 2, ACAN: aggrecan, COMP: cartilage oligomeric matrix protein.