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| Day | Experimental procedure | Analysis |
| −4 | Seeding in 3D-culture | |
| −1 | Medium replacement (w/o FCS) | |
| 0 | Alamar Blue assay; Growth factor treatment | Cell count |
| 3 | Alamar Blue assay; Growth factor treatment | Cell count |
| 5 | Alamar Blue assay; Growth factor treatment | Cell count |
| 7 | Alamar Blue assay;RNA isolation, Real-Time PCRMicroVue C1CP EIA | Cell countGene expressionCol-I protein synthesis |
