FIG. 2.
Sequence-specific inhibition of HIV-1 replication. (A) SupT1 cells stably transduced with the siRNA-Nef vector (left panels) or the empty vector (right panels) were infected with wild-type HIVLAI (closed circles) or HIVrtTA with the Nef gene deleted (open circles). The virus input levels were 800 pg of CA-p24 (top panels), 4,000 pg of CA-p24 (middle panels), and 8,000 pg of CA-p24 (bottom panels) in 5-ml cultures. Virus spread was monitored by determining the CA-p24 level in the culture supernatant. (B) siRNA-Nef and control cells were transfected with 10 μg of DNA encoding wild-type HIVLAI or HIVrtTA with the Nef gene deleted as described previously (7). Virus production in the culture supernatant at 2 and 3 days after transfection was measured by a CA-p24 enzyme-linked immunosorbent assay.