Figure 2. The cross-reactivity and specificity of the anti-peptides antibodies.

A–C. The titers of the antisera against rat/human peptides were determined by an ELISA test in which plates were coated with the same sites human/rat peptides. Complete cross-reactivity of the anti-R32 and anti-R215 antibodies from rat and human sequences was confirmed, while the anti-R72 antibodies showed low cross-reactivity. D. The anti-peptides antibodies were used as primary antibodies, western blotting demonstrated that the anti-peptides antibodies specifically bound to renin.