Table 3. GO term over-representation analysis of DEGs in the gills tissue of mussels exposed to copper along with heat stress.

Condition	Level	Go Term	N	(up)	Gene ID
16_Cu/16°C	6	Translation	16	14	AJ625495, AJ625269, AJ626374, AJ516491, AJ624125, AJ625132, AJ626437, AJ625006, AJ625324, AJ625356, AJ625548, AJ625546, AJ623547, AJ624301, AJ625604, AJ624649
	4	Organ development	7	3	AJ516886, AJ625490, AJ625655, AJ516404, AJ624125, AJ625488, AJ626467
	3	Ribosome biogenesis	7	4	AJ516491, AJ625133, AJ626437, AJ625356, AJ623352, AJ623547, AJ624649
	2	Growth	5	4	AJ516886, AJ516404, AJ626179, AJ626329, AJ623342
	4	Post-embryonic development	5	4	AJ516886, AJ516404, AJ625488, AJ626179, AJ626329
	4	Nervous system development	5	3	AJ516886, AJ625655, AJ516404, AJ624125, AJ625488
	4	Chitin catabolic process	5	0	AJ624093, AJ624637, AJ624087, AJ625051, AJ625569
	3	Cellular macromolecular complex assembly	5	4	AJ626179, AJ626329, AJ625083, AJ516796, AJ516663
	3	Nuclear mRNA splicing, via spliceosome	5	5	AJ516537, AJ516404, AJ626179, AJ626329, AJ625083
20_Cu/20°C	4	Response to stimulus	9	6	AJ624926, AJ625244, AJ625131, AJ623342, AJ625488, AJ625311, AJ624260, AJ624898, AJ625490
	4	RNA processing	5	4	AJ624597, AJ624828, AJ626179, AJ623352, AJ626329
	3	Protein folding	5	3	AJ624926, AJ625244, AJ624969, AJ623698, AJ624898
	4	Multicellular organsmal development	5	2	AJ624597, AJ625441, AJ625488, AJ626179, AJ625655
24_Cu/24°C	6	Translation	41	38	AJ624649, AJ623547, AJ625549, AJ625548, AJ625546, AJ625447, AJ625934, AJ624248, AJ625342, AJ624732, AJ625244, AJ624925, AJ624829, AJ626437, AJ516491, AJ626296, AJ624429, AJ624426, AJ516392, AJ624488, AJ624324, AJ626091, AJ625324, AJ624871, AJ624125, AJ625874, AJ516873, AJ626184, AJ625376, AJ623665, AJ516412, AJ624503, AJ625957, AJ624109, AJ625366, AJ625006, AJ516364, AJ516361, AJ624454, AJ516752, AJ624844
	3	Ribosome biogenesis	12	12	AJ624649, AJ623547, AJ625342, AJ626437, AJ625133, AJ516491, AJ516392, AJ516873, AJ625376, AJ623665, AJ625366, AJ516361
	3	Cellular macromolecular complex assembly	10	6	AJ516796, AJ625595, AJ624686, AJ625091, AJ626329, AJ516582, AJ625866, AJ626179, AJ516600, AJ516663
	3	Microtubule-based movement	7	0	AJ623937, AJ516796, AJ625595, AJ516886, AJ625091, AJ625473, AJ625866
	3	DNA conformation change	7	7	AJ626296, AJ516886, AJ626329, AJ625027, AJ626179, AJ516600, AJ516404

Gene Ontology terms enrichment analysis was carried out comparing the GO term frequency distribution into each condition against that in the whole microarray set (hypergeometric statistics, p,0.05). Only the lowest node per branch of the hierarchical structure of the Gene Ontology that fulfills the filter condition - cut off 5 sequences- was reported. Showed are: experimental condition; Level, level in the GO tee of biological processes; GO Term, over-represented feature; N, number of mussel sequences associated to each GO term; N up, Number of up-regulated genes; Gene ID, EMBL accession number of each sequence found. The over-represented GO terms in copper exposed animals versus relative control (16°C, 20°C and 24°C) (hypergeometric stats, p,0.05).