TABLE 1.

Susceptibility of human cell lines to BoHV-4 infection^a

Group	Source	Cell line	ATCC no.	Origin	BoHV-4 gene expression (%)
					EGFP	MAb 35 α-E/L	MAb 29 α-L
Control	Bovine	MDBK	CCL-22	Kidneyb	71.1	39.8	38.8
Group 1	Human	U-266	ACC 9	Multiple myeloma	1.2	0	0
	Human	U-937	CRL-1593.2	Histiocytic lymphoma	0	0	0
	Human	KARPAS-45	ACC 105	T-cell leukemia	1	0	0
	Human	MOLT-3	CRL-1552	T-cell leukemia	0	0	0
	Human	MOLT-4	CRL-1582	T-cell leukemia	0	0	0
	Human	Jurkat	ACC 282	T-cell leukemia	0	0	0
	Human	L-540	ACC 72	Hodgkin's lymphoma	0	0	0
	Human	KM-H2	ACC 8	Hodgkin's lymphoma	0	0	0
	Human	DOHH-2	ACC 47	B-cell lymphoma	0	0	0
	Human	GRANTA-519	ACC 342	B-cell lymphoma	0	0	0
	Human	NALM-6	ACC 128	B-cell leukemia	0	0	0
	Human	Daudi	CCL-213	Burkitt's lymphoma	0	0	0
	Human	Raji	CCL-86	Burkitt's lymphoma	0	0	0
Group 2	Human	293	CRL-1573	Kidneyc	72	6	3
	Human	MCF7	HTB-22	Breast adenocarcinoma	13.3	2.9	1.5
	Human	MDA-MB-231	HTB-26	Breast adenocarcinoma	19	2.7	0.3
	Human	SK-BR-3	HTB-30	Breast adenocarcinoma	37.2	3	1.2
	Human	Hs 578T	HTB-126	Breast carcinoma	50	5	3.8
	Human	DU-145	ACC 261	Prostate carcinoma	7	2	1
	Human	PC-3	CRL-1435	Prostate adenocarcinoma	10	0.8	0.7
	Human	HeLad	CCL-2	Cervix adenocarcinoma	35	0.2	0.1

^aA total of 21 human cell lines and MDBK cells (used as a positive control) were infected at an MOI of 0.5 PFU/cell with the strain V.test EGFP XhoI of BoHV-4. At 30 h postinfection, the cells were harvested and treated as described in Materials and Methods for the detection of EGFP, MAb 35 epitope, and MAb 29 epitope expressed as IE, E-L, and L proteins, respectively. The numbers indicated in the right three columns represent the percentages of positive cells (n = 10,000 cells). Based on the results obtained, the cell lines can be classified into two groups: group 1 cell lines are resistant or poorly sensitive to BoHV-4 infection. Group 2 cell lines are sensitive but poorly permissive to BoHV-4 infection.

^bThat is, normal epithelial cells.

^cThat is, adenovirus 5 DNA-transformed epithelial cells.

^dHeLa cells were kindly provided by H. Wajant (University of Stuttgart, Stuttgart, Germany).