TABLE 1.
Group | Source | Cell line | ATCC no. | Origin | BoHV-4 gene expression (%)
|
||
---|---|---|---|---|---|---|---|
EGFP | MAb 35 α-E/L | MAb 29 α-L | |||||
Control | Bovine | MDBK | CCL-22 | Kidneyb | 71.1 | 39.8 | 38.8 |
Group 1 | Human | U-266 | ACC 9 | Multiple myeloma | 1.2 | 0 | 0 |
Human | U-937 | CRL-1593.2 | Histiocytic lymphoma | 0 | 0 | 0 | |
Human | KARPAS-45 | ACC 105 | T-cell leukemia | 1 | 0 | 0 | |
Human | MOLT-3 | CRL-1552 | T-cell leukemia | 0 | 0 | 0 | |
Human | MOLT-4 | CRL-1582 | T-cell leukemia | 0 | 0 | 0 | |
Human | Jurkat | ACC 282 | T-cell leukemia | 0 | 0 | 0 | |
Human | L-540 | ACC 72 | Hodgkin's lymphoma | 0 | 0 | 0 | |
Human | KM-H2 | ACC 8 | Hodgkin's lymphoma | 0 | 0 | 0 | |
Human | DOHH-2 | ACC 47 | B-cell lymphoma | 0 | 0 | 0 | |
Human | GRANTA-519 | ACC 342 | B-cell lymphoma | 0 | 0 | 0 | |
Human | NALM-6 | ACC 128 | B-cell leukemia | 0 | 0 | 0 | |
Human | Daudi | CCL-213 | Burkitt's lymphoma | 0 | 0 | 0 | |
Human | Raji | CCL-86 | Burkitt's lymphoma | 0 | 0 | 0 | |
Group 2 | Human | 293 | CRL-1573 | Kidneyc | 72 | 6 | 3 |
Human | MCF7 | HTB-22 | Breast adenocarcinoma | 13.3 | 2.9 | 1.5 | |
Human | MDA-MB-231 | HTB-26 | Breast adenocarcinoma | 19 | 2.7 | 0.3 | |
Human | SK-BR-3 | HTB-30 | Breast adenocarcinoma | 37.2 | 3 | 1.2 | |
Human | Hs 578T | HTB-126 | Breast carcinoma | 50 | 5 | 3.8 | |
Human | DU-145 | ACC 261 | Prostate carcinoma | 7 | 2 | 1 | |
Human | PC-3 | CRL-1435 | Prostate adenocarcinoma | 10 | 0.8 | 0.7 | |
Human | HeLad | CCL-2 | Cervix adenocarcinoma | 35 | 0.2 | 0.1 |
A total of 21 human cell lines and MDBK cells (used as a positive control) were infected at an MOI of 0.5 PFU/cell with the strain V.test EGFP XhoI of BoHV-4. At 30 h postinfection, the cells were harvested and treated as described in Materials and Methods for the detection of EGFP, MAb 35 epitope, and MAb 29 epitope expressed as IE, E-L, and L proteins, respectively. The numbers indicated in the right three columns represent the percentages of positive cells (n = 10,000 cells). Based on the results obtained, the cell lines can be classified into two groups: group 1 cell lines are resistant or poorly sensitive to BoHV-4 infection. Group 2 cell lines are sensitive but poorly permissive to BoHV-4 infection.
That is, normal epithelial cells.
That is, adenovirus 5 DNA-transformed epithelial cells.
HeLa cells were kindly provided by H. Wajant (University of Stuttgart, Stuttgart, Germany).