TABLE 2.
Primers used in this study
| Sequence | GenBank accession no. | Orientation | Primera | Expected size of PCR product (bp) |
|---|---|---|---|---|
| Viral sequences | ||||
| Bo5 | AF318573 | Forward | 5′-GCTACAGAAAATGGCCAGTAAAG-3′ | 1,149 |
| Reverse | 5′-TCATGTCCTGAGTGGGTCTATG-3′ | |||
| ORF22 | AF318573 | Forward | 5′-CCGGGTGAAACAAGTTCCTG-3′ | 563 |
| Reverse | 5′-GGTCAGAGAACATATGATAACATC-3′ | |||
| EGFP | U55762.1 | Forward | 5′-GGAATTCCATGGTGAGCAAGGGCGAGG-3′ | 734 |
| Reverse | 5′-GAAGCTTTTACTTGTACAGCTCGTCCATG-3′ | |||
| Cellular sequence (MCP) | NM_172361 | Forward | 5′-TGTGAGGAGCCACCAACA-3′ | 182 |
| Reverse | 5′-ATAACAGGCGTCATCTGA-3′ |
a
The pairs of primers used in this study were designed based on the sequences available in the GenBank database in order to amplify BoHV-4 or the cellular ORFs listed in the left column.