Figure 4. Generation of a Thioflavin T positive isoform via RT-QuIC occurs faster for moPrP^RL than moPrP^WT.

RT-QuIC reactions containing moPrP^WT (green lines) and moPrP^RL (purples lines) were seeded with brain homogenate from terminally ill 22 L prion infected mice (solid lines) or brain homogenate from mock infected controls (dashed lines) at a dilution of 2×10⁻² (A) or 2×10⁻³ (B). Relative fluorescence over time was averaged for quadruplicate reactions. Time at beginning of conversion determined as the time point at which fluorescence rose from baseline to above 24,000 fluorescence units was compared for both dilutions of 22 L prion seed using GraphPad Prism software (shown in hours; data from 3 independent experiments) (C). Reactions with moPrP^RL as a substrate exhibited earlier conversion to a Thioflavin T positive amyloid structure than reactions with moPrP^WT substrate.