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. 2013 Jun 25;8(6):e66356. doi: 10.1371/journal.pone.0066356

Figure 3. NCTD regulates expression of α-SMA and E-cadherin in HK-2 cells stimulated by TGF-β1.

Figure 3

(a and b) Representative RT-PCR (a) and Western blot (b) results show that NCTD inhibited the expression of α-SMA mRNA and protein in HK-2 cells stimulated by TGF-β1. * P<0.05 vs. negative control group (TGF-β1 0 ng/ml), #P<0.05 vs. positive control group (TGF-β1 5 ng/ml + NCTD 0 µg/ml). (c and d) NCTD increased expression of E-cadherin mRNA and protein in HK-2 cells stimulated by TGF-β1.* P<0.05 vs. negative control group (TGF-β1 0 ng/ml) and #P<0.05 vs. positive control group (TGF-β1 5 ng/ml + NCTD 0 µg/ml). (e) NCTD (2.5 μg/ml) regulated the phenotypic changes in HK-2 cells stimulated with TGF-β1 (Immunofluorescence×200).