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| Gene | Upstream sequence | Downstream sequence |
| α-SMA | 5'-TCC TGA CCC TGA AGT ATC CG-3' | 5'-TCT CCA GAG TCC AGCA CAA T-3' |
| E-cadherin | 5'-GTC AAA CGG CAT CTA AAG C-3' | 5'-CAA AGA CCT CCT GGA TAA ACT-3' |
| TGF-β1 | 5'-CCG CAA CAA CGC AAT C-3' | 5'-ATG AGG AGC AGG AAG GGT -3' |
| β-actin | 5'-ATG AGG AGC AGG AAG GGT -3' | 5'-ACCCAGGAAGGAAGGCT -3' |
