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. 2004 Mar;78(5):2637–2641. doi: 10.1128/JVI.78.5.2637-2641.2003

FIG. 3.

FIG. 3.

Inhibition of HPV-11 in vitro DNA replication by peptides 6N40A and 6N40A dimer. (A) pUC18/7870-99, an in vitro DNA replication template that contains 182 bp (nucleotides 7870 to 0099) from the HPV-11 locus control region inserted into pUC18. (B) NdeI-digested replication products from cell-free replication assays using HPV-11 ORI-containing plasmid (pUC18/7870-99) and increasing concentrations of 6N40A, 6N40A dimer, and E2P. E1, E2, and peptide were added as noted. Lanes corresponding to initial and baseline intensities of counts (I0 and Ibase, respectively) are indicated. (C) Replication assays with increasing peptide concentrations were quantified by both PhosphorImager and filter-binding techniques, and the resulting data for both were fitted to two-parameter hyperbolic decay curves. 6N40A dimer inhibited replication (circles) with an IC50 of 631 nM, while monomeric 6N40A inhibited replication (squares) less efficiently (IC50 = 27.3 μM). Peptide E2P (triangles) did not inhibit in vitro DNA replication at the concentrations tested.