Figure 4. P. brasiliensis Agn1p is a specific endo α-1,3-glucanase.

(A) Inhibition profile of exo-glucoamylase from A. niger (gray) and endo-α-1,3-glucanase from P. brasiliensis (black). Note that none of the indicated inhibitors reduced Agn1p-his activity significantly, even at a high concentration of 250 µM. (B) Agn1p substrate specificity. Purified Agn1p-his was incubated with the indicated substrates at 1 mg/ml. Reactions were carried out at optimal conditions by triplicate.