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. 2013 Jun 25;8(6):e66965. doi: 10.1371/journal.pone.0066965

Figure 5. Time courses of the expression of FcγRs of PMNs after pre-inoculation with PRRSV strains.

Figure 5

PMNs were isolated serially and FcγRs mRNA was measured by real-time PCR, and normalized relative to the expression of GAPDH. Results are shown as mean ± SE (n = 5). A. The expression of FcγRIIB. B. The expression of FcγRIIIA. The formula for calculating FcγRs modulation was Δ2-ΔΔCT: Δ2-ΔΔCT = 2-ΔΔCT inoculation-2-ΔΔCT mock-infected control.2−ΔΔCT = 2-(CT, FcγR-CT, GAPDH) Time x-(CT, FcγR-CT, GAPDH) Time 0. *, p<0.05. **, p<0.01.