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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: J Heart Lung Transplant. 2013 May 2;32(7):714–722. doi: 10.1016/j.healun.2013.04.006

Figure 1.

Figure 1

Isolation of lung infiltrating lymphocytes (LILs) on day 30 from our murine alloimmune model (n=5) following MHC class I Abs administration on day 1, 2, 3, 6 and weekly. (A) MACS beads purified CD3+ T cells with a purity of 94±3%. The CD8+ T cells in the CD3+ fraction amounted for 21±5% (B), and CD4+ T cell fraction amounted for 63±9% (C). The CD3+ T cells were further positively selected for CD8+ (D) and CD4+ (E) T cells with >90% purity. The data were obtained by pooling of LILs from 5 different animals which developed OAD and represented as mean ± SEM.