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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: J Mammary Gland Biol Neoplasia. 2013 Apr 28;18(2):143–147. doi: 10.1007/s10911-013-9282-8

Figure 1.

Figure 1

Expression of Tbx2 and Tbx3 in the developing mammary gland and the effect of heterozygosity for a mutation in Tbx3. A–D. Whole mount in situ hybridization for Tbx2 and Tbx3 at embryonic day (E) 10.5 and E11.5 shows expression of both genes in the mammary line between the forelimb and hindlimb and Tbx3 expression in the mammary placodes when they first appear (arrowheads). E, F. Skin and mammary gland preparation from late gestation embryos stained with Carmine’s Alum to show the nipple and ductal tree, which is reduced in Tbx3 heterozygotes. G, H. Mammary gland preparations from 8 week old virgin females stained with Carmine’s Alum showing a reduction in the branching network of Tbx3 heterozygotes. (Modified with permission from [7]).