FIG. 3.

In vivo viral entry inhibition by soluble nectin-1 as measured by viral load. Progesterone-treated female mice were inoculated intravaginally with HSV-2 wild-type 186 strain (10⁵ PFU/mouse) (A), 186TKΔΚpn (10⁶ PFU/mouse) (B), or HSV-1 wild-type KOS strain (10⁶ PFU/mouse) (C). Prior to inoculation, viruses were treated with soluble recombinant nectin-1 or PBS for 2 h on ice. Vaginal washes were collected at 24 h postinfection, and viral titers were measured by plaque-forming assay. These results are expressed as the viral titer ± standard deviation and are representative of five similar experiments with five mice in each group.