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IR8a binds to IR64a in vivo. A, Schematic drawing of large-scale isolation of antennal tissue for co-IP experiments. B, Silver stain of co-IP proteins from IR64a-HA transgenic flies (HA) and wild-type flies (control). The red arrowhead points to a silver stain positive band that is present in the HA sample but absent in control sample. C, Western blot analysis of independently prepared antennal tissue showing that IR8a is coimmunoprecipitated with IR64a-HA.
