Figure 6.

ERα is required on astrocytes to reduce CCL2 expression within reactive astrocytes. A, CCL2 (red), GFAP (green), and DAPI (blue) are expressed in EAE spinal cord in a WT mouse with EAE. Scale bar, 120 μm. B, CCL2 is coexpressed with GFAP. Scale bar, 27 μm. C, CCL2 is coexpressed with infiltrating immune cells stained with DAPI. Scale bar, 27 μm. D, Treatment with ERα ligand was able to reduce coexpression of CCL2 with GFAP in WT, but not aCKO-ERα, mice with EAE. *p < 0.05 versus WT + No EAE and WT + EAE + ERα ligand (ANOVA with post hoc Bonferroni pairwise analysis). E, Treatment with ERβ ligand was not able to reduce coexpression of CCL2 with GFAP in WT or aCKO-ERβ mice with EAE. *p < 0.05 versus WT + No EAE (ANOVA with post hoc Bonferroni pairwise analysis). n = 5 per group.