Table 1.

Primer sets used for PCR amplification of Sorghum SUTs. Restriction site sequences are underlined.

Gene	Forward primer (5′–3′)	Reverse primer (5′–3′)
Full-length primers
SbSUT1	GTCGTCCCGTACGTGTGC	ATCTTGCACGGTTGGGTTT
SbSUT2	CCGCAGCGACACCTACAC	AATGGCAAAATGGGGCTAAGT
SbSUT3	CTCCACACCTCTCCGGTTT	CGACAGTAGTGGTTGATCG
SbSUT4	TCAAAGCAACTCAGCGATTC	AGCTGCAACTCTTCCAAAGC
SbSUT5	GTAGCCATGGACGGTGGTG	CCGCCTGGCGATAGATAGAT
SbSUT6	CGTTCCTGCTCCTCTCACTC	TGGATTTCCGATCATCCACT
Restriction cloning primers
SbSUT1	CTCGCGGAATTCATGGCTCGCGGCGA	GGCCGTGTCGACTCAGTGGCCGCCCG
	EcoRI	sall
SbSUT2	GGCGCGGTCGACATGGACGCCGGCACC	TTGGGCAGTCGACTCAGCCAAATCCATGG
	sall	XhoI
SbSUT3	CCGGTTGAATTCATGGCTGCTGATGGC	CTGGACCTCGAGTCAATGGCCTCCTC
	EcoRI	XhoI
SbSUT4	CCGTGAGAATTCATGCCGCCGCGCAC	GTAATGGTCGACATTATCGGTGCGTGC
	EcoRI	sall
SbSUT5	AATTCGAGCGGCCGCATGGACGGTGGTGAC	GCGATAGGATCCTCAGTGGCCGCCGC
	NotI	BamHI
SbSUT6	GCCCGGCGGCCGCATGGACGACGGTGAC	CCTGGAGGATCCTCAACAGTGGCCGC
	NotI	BamHI
qPCR primers
SbSUT1	GTGCTCCTGTAATCTTTGTGTCC	ACTATACTGCACATTGATTGATCG
SbSUT2	GCACATGCATTGAATGAACC	TTCGCATTTGGAAATTCCTC
SbSUT3	GGCCGGATCAAACAAGAT	GGCATTGCGAAGGAATGA
SbSUT4	CGATCCATGATGATGTCCAG	GTTCCAGGCCTTGCTGTC
SbSUT5	CCCGTAGTGTTGCGGAGTC	CCAATGGATCGGAAAATAAAG
SbSUT6	GCACAACAGCACAAAGAAGG	AGGCAGAAGAGGCTGAGATG
SbGAPDH	AGGGTATCATGGGCTACGTG	AGTTGTCGTTCAGGGCAATC
SbEF1a	CATGGTGGTGGAGACCTTCT	TCCTTCTTCTCCACGCTCTT