Table 3.

Flow cytometry analysis of Tie2-GFP expression in peripheral blood mononuclear cells (PBMCs) derived from Tie2-GFP mice fed a high-fat or chow diet for 8 weeks.

	CD11b+ (% of CD45+)	CD11b+/CD115+ (% of CD45+)	GFP+ (% of CD45+/CD11b+)	GFP+ (% of CD45+/CD11b+/CD115+)
Chow	30 ± 4	9 ± 2	0.6 ± 0.1	0.8 ± 0.3
HFD	33 ± 6	11 ± 3	0.6 ± 0.2	0.8 ± 0.5

PBMCs were isolated and stained for CD45, CD11b, and CD115. The population positive for the common leukocyte antigen, CD45, was analyzed for CD11b⁺ myeloid cells and CD11b⁺/CD115⁺ monocytes. The percentage of these populations appearing to have a positive signal for GFP is shown. Data is presented as mean ± SD (n = 3).