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. 2013 Jun 11;2013:870472. doi: 10.1155/2013/870472

Figure 4.

Figure 4

Chloroquine treatment attenuates the cellular HO-1 and iron metabolism response to Hb:Hp exposure. (a) Relative mRNA (left) and protein (right, Western blot) expression levels of HO-1 in chloroquine-treated and untreated CD163-positive HEK cells after stimulation with Hb:Hp (2 mg/mL) for 8 hours (values represent mean ± SEM of at least three independent experiments). (b) Relative mRNA (left) and protein (right, Western blot) expression levels of HO-1 in human monocyte derived macrophages after Hb:Hp (2 mg/mL) exposure for 8 hours in the presence or absence of chloroquine. (c) Relative changes of transferrin receptor (TfR1) and ferroportin mRNA expression in human monocyte derived macrophages after Hb:Hp (2 mg/mL) exposure for 8 hours in the presence or absence of chloroquine. All mRNA changes are given relative to control cells (data indicate mean ± SEM of at least three independent experiments).