Figure 1.

The mRNA and protein of Wnt5a and Fzd2 receptor in the spinal cord of adult amyotrophic lateral sclerosis (ALS) mice and wild-type (WT) littermates at 95, 108, and 122 days of age. A. The gene expression of Wnt5a was higher in ALS mice than that in WT mice at each age. Beta-actin was used for normalization. B. Representative immunoblot showing that Wnt5a expression was greater in ALS mice than that in WT mice at each age. GAPDH was used for normalization. C. Densitometric analysis of the RT-PCR results shown in panel (A). Relative amounts of Wnt5a were calculated as the ratio of Wnt5a to beta-actin densities (n = 6). D. Densitometric analysis of the immunoblot shown in panel (B). Relative amounts of Wnt5a were calculated as the ratio of Wnt5a to GAPDH densities (n = 6). E. The gene expression of Fzd2 was greater in ALS mice at each age than that in WT mice. Beta-actin was used for normalization. F. Representative immunoblot showing that Fzd2 expression was greater in ALS mice at each age than that in WT mice. GAPDH was used for normalization. G. Densitometric analysis of RT-PCR shown in panel (E). Relative amounts of Fzd2 were calculated as the ratio of Wnt5a to beta-actin densities (n = 6). H. Densitometric analysis of the Western blot shown in panel (F). Relative amounts of Fzd2 were calculated as the ratio of Fzd2 to GAPDH densities (n = 6).