Figure 2.

Ibuprofen and the related 2-arylpropanoic acids are inhibitors of slow-growing Mycobacterium. (A) Chemical structures of commercially available compounds 1–9 and synthetically modified-IBP derivatives 10–16. (B) Growth curve of M bovis BCG in liquid media growing with 1×minimum inhibitory concentration (MIC) and 2×MIC of 1, 7 and 15 added before inoculation (as shown by the arrow). (C) Growth curve describing the same experiment with addition of the inhibitors at an early-exponential phase (as shown by the arrow). (D) Colony forming units (CFU) count of M bovis BCG cells treated with 1×MIC and 2×MIC of 1, 7 and 15 added before inoculation. CFU was determined simultaneously for all treatment when the OD600 of the control was 0.8. (E) CFU count determined simultaneously for all treatments 24 h after the addition of the inhibitors at the early-exponential phase.