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. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: J Struct Funct Genomics. 2008 Dec 12;10(1):57–66. doi: 10.1007/s10969-008-9053-8

Figure 2.

Figure 2

Negative stain EM Field: GroEL complexes A) Electron Micrograph of the PEPCK-GST sample eluted with glutathione clearly shows the presence of GroEL and additional density at the protein substrate binding site.. Inset: GroEL after treatment with ATP removes the complexed protein. B) Initial correspondence analysis of negatively stained GroEL-glutamine synthetase samples (20,21) revealing uncomplexed GroEL and GroEL complexed with protein is compared with a similar analysis from the current study showing uncomplexed GroEL and GroEL complexed with protein, presumed to be GroEL-PEPCK-GST. Arrow points to area of increased electron density in current study. For GroEL-PEPCK-GST, the distribution of images in a typical population was ~ 60% free, 40% bound) see methods for more details).