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. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: J Struct Funct Genomics. 2008 Dec 12;10(1):57–66. doi: 10.1007/s10969-008-9053-8

Figure 5.

Figure 5

The column based refolding and regain of GST- PEPCK activity was compared with the intrinsically bound GroEL and the GST-PEPCK bound sample that contained an extra bolus of GroEL added to the column slurry (~ 1 mg/ml or ~ 1.2 uM GroEL oligomer). After ATP and glycerol addition, the refolded proteins and GroEL were eluted from the column by adding glutathione. As is readily apparent, the sample with the extra GroEL added exhibited higher PEPCK activity. In both cases, with either intrinsic GroEL or extra GroEL present, the PEPCK activities begin to show a decline after 2 hours.