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Demonstration of the partitioning of a Native folded Protein onto GroEL beads – Malate dehydrogenase. Panel A. Immobilization scheme for GroEL shows that orientation will not matter. A single or double binding site will always be presented. Panel B. After a temperature pulse of 50°C for 10 min, activity of MDH decreases to zero within ten minutes (partitioning phase). After the temperature is returned to 37°C, glycerol and ATP facilitate refolding of partitioned malate dehydrogenase.
