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. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: J Struct Funct Genomics. 2008 Dec 12;10(1):57–66. doi: 10.1007/s10969-008-9053-8

Figure 8. Partitioning reaction of MDH in the absence and presence of the stabilizers trehalose/sucrose.

Figure 8

In the absence of stabilizing osmolytes (closed triangles), the thermally denatured MDH will partition onto the immobilized GroEL support. If thermal denaturation occurs in the presence of stabilizers such as a trehalose/sucrose mixture, a substantial amount of MDH activity remains in solution and is readily detectable (closed circles). After 20 min at 46°C, 70 percent of the original activity of MDH (double arrow) remains in solution while the unprotected sample is no longer active or detected in the assay solution.