Skip to main content
. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: J Vaccines Vaccin. 2011 Dec 2;2(4):1000124. doi: 10.4172/2157-7560.1000124

Figure 4. Ad5-HIVBr18 immunization induces higher proliferative responses up to 28 weeks after the last immunization.

Figure 4

BALB/c mice were immunized as described earlier. Twenty-eight weeks after the last immunization, splenocytes from immunized mice (6 per group) were pooled, labeled with CFSE (1.25μM) and cultured for 5 days, in the presence of 5μM of pooled HIV-1 peptides. Cells were analyzed by flow cytometry and CFSE dilution on gated CD3+CD4+ or CD3+CD8+ cells was used as readout for antigen-specific proliferation. Experimental groups presented proliferation above cutoff values in all time points. The average of proliferation on splenocytes from pVAX1 and/or Ad5-βgal immunized group was subtracted from the respective experimental groups. DNA= HIVBr18; Ad5= adenovirus 5 encoding HIVBr18. DNA vs Ad5 (2x, SC), p<0.001.