Social behavior of pnd70 offspring. Three-chamber mouse social behavior testing was performed. BALB/c mice of the same age and sex were used as stimulus mice. A 20-min test was performed in which only the behavior in the second 10min was analyzed. The behavioral index is the time (s) in the side chamber with the novel mouse of same sex minus time in the side chamber with the novel object for the SFvF1 offspring (A) and the FvSF1 offspring (B). Three-way ANOVA (sex, treatment, and strain) indicated a significant sex difference (F
(1,42) = 30.68, p < 0.001), HgCl2 effect (F
(1,42) = 12.57, p < 0.001) and a significant interaction (p = 0.012) between sex and strain. Pairwise multiple comparisons (Holm-Sidak method) indicated significant differences for the following: H2O versus HgCl2 for males (p = 0.047) and females (p = 0.005), male versus female for H2O (p = 0.002) and HgCl2 (p < 0.001), female SFvF1 versus female FvSF1 (p = 0.013), male versus female FvSF1 (p = 0.45), male versus female SFvF1 (p < 0.001), and H2O versus HgCl2 for SFvF1 offspring (p < 0.001). Number litters assayed for the treatment groups (H2O; Hg) were M (6; 7) and F (5; 7) SFvF1, M (7; 6) and F (6; 6) FvSF1; * indicates significant sex and treatment difference and ** indicates only sex difference.