Skip to main content
. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: ACS Chem Biol. 2012 Dec 18;8(1):46–57. doi: 10.1021/cb300607e

Figure 1.

Figure 1

Chemo-proteomic methods for enriching palmitoylated proteins. (A) Acyl Biotin Exchange (ABE) describes the chemical hydrolysis and capture of thioesterified cysteines(14). The modified Acyl-RAC method uses activated thiol resin(24), eliminating need for additional biotin capture steps. The thiol “cap” can be maleimide, iodoacetamide, or MMTS. (B) Bioorthogonal metabolic labeling describes the endogenous labeling of native palmitoylated proteins with the alkynyl palmitate analogue 17-octadecynoic acid (17-ODYA). After metabolic labeling, cells are lysed and conjugated to biotin-azide using click chemistry for streptavidin enrichment(26).