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. Author manuscript; available in PMC: 2013 Jun 26.
Published in final edited form as: J Cell Physiol. 2009 Jun;219(3):634–641. doi: 10.1002/jcp.21708

Figure 4.

Figure 4

Effect of BRCA1a and BRCA1b on GAL-4-ELK-1 transcriptional activation. (a) MCF7 (b) HLR-ELK-1 cells were plated at a density of 6×105 cells per well in 6-well plates. Cells were cotransfected with 1 μg each of GAL-4-Elk-1, MEK, E1bLuc and pCMV βgal. Cells were also transfected with various concentrations of BRCA1a/BRCA1b, BRCA1a/BRCA1b Y1853 ter mutants and pcDNA3 vector using Lipofectamine 2000 (Invitrogen) according to manufacturer's instructions. DNA was held constant at 5 μg using Gem3. Forty-eight hours after transfections, cells were harvested and assayed for luciferase activity using a luciferase assay system (Promega). Transfections were normalized for β-galactosidase activity. Values represent relative luciferase activity as percent of pcDNA3 vector control.