Figure 3.

Survival of SWR/J mice infected with Mycobacterium tuberculosis (M. tuberculosis) and treated with the ID93/GLA-SE vaccine and reduced antibiotic chemotherapy. SWR/J mice were infected with an LDA of M. tuberculosis H37Rv. Fifteen days later, mice were treated for 60 or 90 days with antibiotics (Rx 60d and Rx 90, respectively). After the completion of the 60-day antibiotic regimen, mice were immunized 3 × 3 weeks apart with ID93/GLA-SE. (A) Protection was assessed by monitoring animal deaths (7 mice/group) due to M. tuberculosis over time. P < .05 (log rank test) is considered to be statistically significant. (B–M) Histopathological evaluation of lung tissues after challenge with M. tuberculosis H37Rv. Inflammatory responses and granuloma (g) formation are shown in H&E sections (B–I), and the presence of AFB (arrows) (J–M) was evaluated. (B, F and J) Mock-treated mice, day 106; (C, J and K) 90-day antibiotic therapy, day 106; (D, H and L) 90-day antibiotic therapy + ID93/GLA-SE, day 241; (E, I and M) 60-day antibiotic therapy + ID93/GLA-SE, day 295 Data shown are representative of 5 mice/group. One representative of 3 experiments is shown.