Figure 5.

Phosphoinositide 3-kinase (PI3K) or extracellular-signal-related kinase (ERK) inhibition abrogated CpG oligodeoxynucleotide (CpG-ODN)–induced attenuation of cardiac dysfunction in polymicrobial sepsis. Mice were treated with the PI3K-specific inhibitor Ly294002 (LY) or the ERK-specific inhibitor U0126 (U0) 15 minutes prior to CpG-ODN administration. Cardiac function was measured by echocardiography before and 6 hours after cecal ligation and puncture. Hearts were harvested, and cellular proteins were isolated for analysis of phosphorylation of Akt (p-Akt; A) and phosphorylation of ERK (p-ERK; B) by Western blotting. Representative blots are shown above in the graph. There were 6 mice in each group. *P < .05. Abbreviations: LY+CpG, CpG-ODN+LY294002; Unt, untreated; U + CpG: CpG-ODN+U0126.