Fig. 5.

In vivo activation of the human CYP2B6 promoter-luciferase reporter construct in mouse liver after vehicle or OPZ administration. Male C57Bl/6 mice were injected with 10 μg of naked plasmid DNA in a rapid (5-s) tail vein injection in sterile saline in a volume equal to 10% of body weight. Following a 16-h recovery period, animals were anesthetized with a solution of ketamine (72 mg/kg) and xylazine (6 mg/kg) and injected with luciferin i.p. At time 0, mice were injected with corn oil vehicle (5 ml/kg, i.p.) or OPZ (150 mg/kg). Images were collected at 0 and 24 h following vehicle or OPZ administration.