Figure 3. Cell surface levels of CD36 are elevated on peritoneal macrophages lacking ADAM17, and maximal expression of CD36 on WT macrophages is during the resolution phase of peritonitis.
WT resident peritoneal macrophages (Res) or peritoneal macrophages from WT or Adam17-null hematopoietic chimera mice were harvested at different times after thioglycollate injection and immediately stained with antibodies to the indicated apoptotic cell receptors and evaluated by flow cytometry. A. Levels of different apoptotic cell receptors were evaluated 96 hours after thioglycollate. B. Histograms following staining of resident peritoneal macrophages or peritoneal macrophages 48, 96 and 120 hours after thioglycollate with anti-CD36 or isotype control (n=3-4 mice/time point). C. The mean fluorescent index (MFI) of CD36 is indicated for F4/80+ macrophages that are CD36+. D. The % of F4/80+ cells expressing CD36 is shown. Numbers within bars indicate the number of mice evaluated, and p values for significant differences are shown.